Single-primer PCR correction: a strategy for false-positive exclusion

Biologically-generated primer for PCR: PCR primer of unknown sequence

We describe a method for producing specific PCR primers directly from PCR product, bypassing the usual need to know the primer sequence. Lack of abundance of primers derived from a PCR product is compensated for by the incorporation of an arbitrary 5'TAG sequence which acts as a surrogate template target for the bulk amplification phase. We use the technique to amplify clonospecific rearranged ...

The Effect of False Correction Strategy and Inference Strategy on the Paramedical Students’ Reading Comprehension and Attitude

There is a bulk of studies supporting the positive effect of strategy instruction on reading comprehension. This study examined the effect of two reading strategies (i.e., false correction and inference strategy) on English reading comprehension of Iranian paramedical students, using a pretest, posttest, control group design. It also surveyed their attitudes toward the effect and usefulness of ...

A Simple Genome Walking Strategy to Isolate Unknown Genomic Regions Using Long Primer and RAPD Primer

Background: Genome walking is a DNA-cloning methodology that is used to isolate unknown genomic regions adjacent to known sequences. However, the existing genome-walking methods have their own limitations. Objectives: Our aim was to provide a simple and efficient genome-walking technology. Material and Methods: In this paper, we dev...

a novel single step double positive double negative selection strategy for β-globin gene replacement

introduction: beta thalassemias are a heterogenous group of autosomal recessive disorders, characterized by reduced or absent production of the b-globin chain by the affected allele. transplantation of allogenic hematopoietic stem cells (hsc) is a curative approach but this therapeutic option is not available to the majority of patients. transplantation of genetically corrected autologous hsc i...

false positive pcr for herpes simplex virus (hsv) in childhood cns infections